Protein engineering by rational design and directed evolution

2018-05-29T04:14:19Z (GMT) by Thomas Shafee
A 2-hour lecture summarising key principles, methods and applications of protein engineering techniques. Illustrated with examples from medicine, agriculture, research, bioenergy, and industry. The material is split into two main sections.

1) Rational design - including manual, bioinformatic and computational techniques for protein modification and de novo design.
2) Directed evolution - covering different systems for generating genetic diversity, selection and screening systems, and linking phenotype to genotype.

Finally some hybrid techniques that combine the two approaches are briefly explained.